![]() The remaining ~99% either fail to grow on the synthetic growth media used in the laboratory or require special or unidentified culture techniques 3. ![]() About 10 6 prokaryotic species were estimated to exist on Earth 1, of which ~12,000 (~1% of the total) species have so far been cultivated and validly described 2. These findings suggested that limited knowledge on the interaction of microbes provide threshold to traditional isolation method.Įarth is by large a microbial world. Metabolomes studies indicated that the spent-culture supernatant comprises several low-molecular-weight organic substrates that can be utilized as potential nutrients for the growth of these bacteria. 16S rRNA gene fingerprinting characterized majority of the colonies isolated from these media as previously uncultivated Chloroflexi, of which 36 are potential novel species (16S rRNA sequence identity <98.5%). strain SYSU G00190W was prepared for targeted isolation of Chloroflexi in the Tibet and Yunnan hot spring samples. Exploiting this result, an ameliorated isolation medium containing 10% spent-culture supernatant of Tepidimonas sp. strain SYSU G00190W enhanced the growth of Chloroflexus sp. A preliminary growth-promotion assay determined that Tepidimonas sp. We analyzed the entire co-occurrence networks between the OTUs of Tibet and Yunnan hot spring samples, and found that less abundant OTUs such as genus Tepidimonas (relative abundant <1%) had high-degree centricity (key nodes), while dominant OTUs particularly genus Chloroflexus (relative abundant, 13.9%) formed the peripheral vertexes. The perplexity of the complex multispecies community interactions is one of the many reasons why majority of the microorganisms are still uncultivated.
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